1EXF

EXFOLIATIVE TOXIN A

Experimental procedure

Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1995-11-15
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	71.500, 71.500, 122.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.000 - 2.100
R-factor	0.172
Rwork	0.172
R-free	0.20400
RMSD bond length	0.006
RMSD bond angle	1.090 *
Data reduction software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	17.000
High resolution limit [Å]	2.100
Rmerge	0.072
Number of reflections	16869
Completeness [%]	87.7
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	cacodylate	100 (mM)
3	1	drop	PEG8000	29 (%)
4	1	drop	ammonium sulfate	200 (mM)
5	1	reservoir	cacodylate	100 (mM)
6	1	reservoir	PEG8000	29 (%)
7	1	reservoir	ammonium sulfate	200 (mM)