1EX5

FRUCTOSE 1,6-BISPHOSPHATE ALDOLASE FROM RABBIT MUSCLE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1994
Detector	SIEMENS HI-STAR
Spacegroup name	P 1 21 1
Unit cell lengths	165.968, 58.364, 86.954
Unit cell angles	90.00, 103.16, 90.00

Refinement procedure

Resolution	9.000 - 2.200
R-factor	0.178 *
Rwork	0.179
R-free	0.22900
RMSD bond length	0.011 *
RMSD bond angle	1.812 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	9.000
High resolution limit [Å]	2.150	2.150
Rmerge	0.077 *
Total number of observations	88801 *
Number of reflections	56675
Completeness [%]	81.0 *	98
Redundancy	1.88	2.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.4	293	40% ammonium sulphate, 5mM EDTA, pH 7.4, SMALL TUBES, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	40 (%sat)
2	1	drop	protein	10 (mg/ml)
3	1	reservoir	ammonium sulfate	50 (%sat)