1EWZ
CRYSTAL STRUCTURE OF THE OXA-10 BETA-LACTAMASE FROM PSEUDOMONAS AERUGINOSA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.415, 82.395, 101.488 |
| Unit cell angles | 90.00, 95.40, 90.00 |
Refinement procedure
| Resolution | 31.220 - 2.400 |
| R-factor | 0.187 * |
| Rwork | 0.187 |
| R-free | 0.25400 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.220 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.057 | 0.067 |
| Number of reflections | 41455 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 98.3 | 91.7 |
| Redundancy | 5.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.5 | 277 | ammonium sulfate, Tris, pH 8.5, EVAPORATION, temperature 277K |
