1EWX

Crystal structure of native tryparedoxin I from Crithidia fasciculata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-07-06
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	37.940, 51.390, 71.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.900 - 1.700
R-factor	0.168
Rwork	0.168
R-free	0.21300 *
RMSD bond length	0.021
RMSD bond angle	0.036
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.200	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.073	0.089
Number of reflections	15563
<I/σ(I)>	5.9
Completeness [%]	97.9	100
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.7	294 *	Kalisz, H.M., (1998) Acta Crystallogr., Sect.D, 55, 696. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	13.5 (mg/ml)
2	1	reservoir	PEG4000	33 (%(w/w))
3	1	reservoir	Tris-HCl	0.1 (M)	pH8.7
4	1	reservoir	sodium acetate	50 (mM)