1EWT
CRYSTAL STRUCTURE OF METABOTROPIC GLUTAMATE RECEPTOR SUBTYPE 1 LIGAND FREE FORM I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-14 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 111.436, 111.436, 293.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.700 |
| R-factor | 0.244 * |
| Rwork | 0.244 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ewk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 3.830 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.146 | 0.520 |
| Total number of observations | 119429 * | |
| Number of reflections | 19643 | |
| <I/σ(I)> | 11.6 | 3.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.08 | 5.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | AMMONIUM SULFATE, TRIS-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium sulfate | 1.9 (M) | |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | drop | Tris-HCl | 200 (mM) |
