1EWT

CRYSTAL STRUCTURE OF METABOTROPIC GLUTAMATE RECEPTOR SUBTYPE 1 LIGAND FREE FORM I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-11-14
Detector	RIGAKU RAXIS IV
Spacegroup name	P 41 21 2
Unit cell lengths	111.436, 111.436, 293.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 3.700
R-factor	0.244 *
Rwork	0.244
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ewk
RMSD bond length	0.013
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	3.830
High resolution limit [Å]	3.700	3.700
Rmerge	0.146	0.520
Total number of observations	119429 *
Number of reflections	19643
<I/σ(I)>	11.6	3.5
Completeness [%]	99.9	100
Redundancy	6.08	5.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	AMMONIUM SULFATE, TRIS-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	1.9 (M)
2	1	drop	protein	10 (mg/ml)
3	1	drop	Tris-HCl	200 (mM)