1EWR
CRYSTAL STRUCTURE OF TAQ MUTS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.734, 96.734, 427.126 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.940 - 3.190 |
| R-factor | 0.331 |
| Rwork | 0.331 |
| R-free | 0.36100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.250 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.067 | 0.443 |
| Number of reflections | 30381 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 88.6 | 85.3 |
| Redundancy | 4.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 27 * | PEG 400, ammonium sulfate, copper chloride, HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 10 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 11 | 1 | reservoir | 1.5 (mM) | ||
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | d | EDTA | 0.1 (mM) | |
| 6 | 1 | drop | 5 (mM) | ||
| 7 | 1 | drop | glycerol | 5 (%) | |
| 8 | 1 | reservoir | HEPES | 90 (mM) | |
| 9 | 1 | reservoir | PEG400 | 7.2 (%) |
