1EWO

THE CYSTEINE PROTEASE CRUZAIN BOUND TO WRR-204

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-10-14
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	45.206, 51.551, 45.511
Unit cell angles	90.00, 116.13, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.221
Rwork	0.221
R-free	0.26200
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.077	0.197
Number of reflections	10544
<I/σ(I)>	7.2
Completeness [%]	92.2	90.6
Redundancy	2.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	0.8M NaCitrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K