1EWK

CRYSTAL STRUCTURE OF METABOTROPIC GLUTAMATE RECEPTOR SUBTYPE 1 COMPLEXED WITH GLUTAMATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-23
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	83.375, 95.219, 97.451
Unit cell angles	90.00, 114.85, 90.00

Refinement procedure

Resolution	17.000 - 2.200
R-factor	0.196 *
Rwork	0.196
R-free	0.22700
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.045	0.137
Number of reflections	68771
<I/σ(I)>	17.5	8.6
Completeness [%]	98.9	96.2
Redundancy	3.26	2.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 4000, HEPES-NaOH, glutamate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000	20 (%)
2	1	drop	L-glutamate	1 (mM)
3	1	drop	protein	10 (mg/ml)
4	1	drop	HEPES-NaOH	200 (mM)