1EWK
CRYSTAL STRUCTURE OF METABOTROPIC GLUTAMATE RECEPTOR SUBTYPE 1 COMPLEXED WITH GLUTAMATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-23 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.375, 95.219, 97.451 |
| Unit cell angles | 90.00, 114.85, 90.00 |
Refinement procedure
| Resolution | 17.000 - 2.200 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.045 | 0.137 |
| Number of reflections | 68771 | |
| <I/σ(I)> | 17.5 | 8.6 |
| Completeness [%] | 98.9 | 96.2 |
| Redundancy | 3.26 | 2.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, HEPES-NaOH, glutamate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG4000 | 20 (%) | |
| 2 | 1 | drop | L-glutamate | 1 (mM) | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | HEPES-NaOH | 200 (mM) |
