1EWJ

CRYSTAL STRUCTURE OF BLEOMYCIN-BINDING PROTEIN COMPLEXED WITH BLEOMYCIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-03-20
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	115.270, 117.000, 79.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.218
Rwork	0.218
R-free	0.30200
RMSD bond length	0.007
RMSD bond angle	1.200 *
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.750
High resolution limit [Å]	2.500	2.500
Rmerge	0.125	0.372
Total number of observations	62934 *
Number of reflections	27188
<I/σ(I)>	4.06
Completeness [%]	70.1 *	56.9
Redundancy	2.03

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	25 *	PEG 8000, ammonium sulfate, magnesium chloride, 2-(N-morpholino)-etanesulfonic acid, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	reservoir	PEG6000	15 (%)
3	1	reservoir	ammonium sulfate	0.1 (M)
4	1	reservoir	magnesium chloride	0.02 (M)
5	1	reservoir	MES-NaOH	0.05 (M)