1EWE

Fructose 1,6-Bisphosphate Aldolase from Rabbit Muscle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1995-11-07
Detector	SIEMENS HI-STAR
Spacegroup name	P 1 21 1
Unit cell lengths	164.240, 57.410, 85.050
Unit cell angles	90.00, 102.65, 90.00

Refinement procedure

Resolution	12.000 * - 2.600
R-factor	0.177 *
Rwork	0.166
R-free	0.23200 *
RMSD bond length	0.011 *
RMSD bond angle	1.800 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.570
High resolution limit [Å]	2.600
Rmerge	0.100 *
Total number of observations	87439 *
Number of reflections	48198 *
Completeness [%]	84.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.4	293	Ammonium sulfate, 43 percent 5mM EDTA, pH 7.4, SMALL TUBES, temperature 293K
1	unknown *	7.4	293	Ammonium sulfate, 43 percent 5mM EDTA, pH 7.4, SMALL TUBES, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	40 (%sat)
2	1	drop	protein	10 (mg/ml)
3	1	reservoir	ammonium sulfate	50 (%sat)