1EWC
CRYSTAL STRUCTURE OF ZN2+ LOADED STAPHYLOCOCCAL ENTEROTOXIN H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-06 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 86.380, 86.380, 228.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.000 - 1.950 |
| R-factor | 0.23 * |
| Rwork | 0.230 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1enf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.700 * |
| Data reduction software | TRUNCATE |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.082 * | 0.246 * |
| Number of reflections | 22772 | |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 92.4 | 94.9 |
| Redundancy | 4.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | 298 | 20% iso-propanol, 100 mM sodium acetate pH 4.6, 200 mM CaCl2, EVAPORATION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | isopropanol | 20 (%(v/v)) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 4 | 1 | reservoir | 200 (mM) |
