1EW6

THE CRYSTAL STRUCTURE AND AMINO ACID SEQUENCE OF DEHALOPEROXIDASE FROM AMPHITRITE ORNATA INDICATE COMMON ANCESTRY WITH GLOBINS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-10-12
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	68.500, 68.400, 61.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.780
R-factor	0.197 *
Rwork	0.197
R-free	0.22500
RMSD bond length	0.005 *
RMSD bond angle	1.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.780
Rmerge	0.051
Total number of observations	66563 *
Number of reflections	24637
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	277 *	Zhang, E., (1996) Acta Crystallogr., D52, 1191. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	ammonium sulfate	200 (mM)
3	1	reservoir	PEG8000	30 (%)