1EW4
CRYSTAL STRUCTURE OF ESCHERICHIA COLI CYAY PROTEIN REVEALS A NOVEL FOLD FOR THE FRATAXIN FAMILY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-25 |
| Detector | FUJI |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 44.178, 44.178, 98.286 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.130 - 1.400 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21300 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.041 | 0.399 |
| Total number of observations | 204695 * | |
| Number of reflections | 22616 * | |
| <I/σ(I)> | 46 | |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.12 | 296 | 0.1 M sodium acetate, 34% PEG 4000, pH 5.12, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | drop | magnesium chloride | 2.0 (M) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 34 (%(w/v)) |
