1EVV

CRYSTAL STRUCTURE OF YEAST PHENYLALANINE TRANSFER RNA AT 2.0 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	1999-04-20
Detector	ADSC
Spacegroup name	P 1 21 1
Unit cell lengths	55.252, 32.988, 61.882
Unit cell angles	90.00, 90.38, 90.00

Refinement procedure

Resolution	25.790 - 2.000
R-factor	0.227
Rwork	0.227
R-free	0.26300
Starting model (for MR)	1tra
RMSD bond length	0.009
RMSD bond angle	12.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.800	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.036 *	0.171 *
Total number of observations	30638 *
Number of reflections	13678
<I/σ(I)>	14.8	2.2
Completeness [%]	88.6	70.7
Redundancy	3.1	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.5	4 *	CRYSTALS GROWN AT 277 K IN 0.010 M TRIS-HCL PH 7.5, 0.012 M MGCL2, 0.002 M SPERMINE, 6% 1,6-HEXANEDIOL. CRYOPROTECTION CONDITIONS: AFTER WASHING IN FRESH CRYSTALLIZATION SOLUTION FOR 10 MIN AND ADDITION OF 30% MPD (V/V), CRYSTALS WERE FLASH-FROZEN IN LIQUID NITROGEN, DIALYSIS, temperature 277.15K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	TRIS-HCL
2	1	1	MGCL2
3	1	1	SPERMINE
4	1	1	1,6-HEXANEDIOL
5	1	2	MGCL2
6	1	2	1,6-HEXANEDIOL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1		tRNA	4 (mg/ml)
2	1	2	Tris-HCl	10 (mM)
3	1	2		12 (mM)
4	1	2	spermine	2 (mM)
5	1		1,6-hexanediol	6 (%(v/v))