1EVU
HUMAN FACTOR XIII WITH CALCIUM BOUND IN THE ION SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.172, 70.764, 133.822 |
| Unit cell angles | 90.00, 106.11, 90.00 |
Refinement procedure
| Resolution | 20.660 - 2.010 |
| Rwork | 0.208 |
| R-free | 0.26600 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 27.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.660 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.043 | 0.669 |
| Number of reflections | 111498 | |
| Completeness [%] | 91.5 | 76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | 294 | 24% 1,2-propanediol, 100 mM sodium potassium phosphate buffer. To incorporate Calcium, the crystals were transferred to 24% 1,2-propanediol and MES buffer and soaked for 1-2 days in 90 mM CaCl2, pH 6.2, VAPOR DIFFUSION, temperature 294K |
