1EVP
CRYSTAL STRUCTURE OF THE CHIMERICAL DECAMER D(CCACTAGTG)R(G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-07-15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.900, 45.760, 49.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.22800 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 2.000 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.031 | |
| Number of reflections | 4634 | |
| Completeness [%] | 87.4 | 67.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 298 | MGCL2, SPERMINE, MPD, SODIUM CACODYLATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MGCL2 | ||
| 2 | 1 | 1 | SPERMINE | ||
| 3 | 1 | 1 | SODIUM CACODYLATE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligomer | 2 (mM) | |
| 2 | 1 | drop | spermine tetrachloride | 50 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | Na cacodylate | 20 (mM) | |
| 5 | 1 | drop | 10 (%) | ||
| 6 | 1 | reservoir | MPD | 60 (%) |
