1EVJ

CRYSTAL STRUCTURE OF GLUCOSE-FRUCTOSE OXIDOREDUCTASE (GFOR) DELTA1-22 S64D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-09-08
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1
Unit cell lengths	47.710, 91.460, 98.920
Unit cell angles	64.53, 84.45, 75.28

Refinement procedure

Resolution	39.140 - 2.700
R-factor	0.241
Rwork	0.241
R-free	0.28400
RMSD bond length	0.007
RMSD bond angle	23.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.083	0.360
Number of reflections	38847
<I/σ(I)>	14.6	2.8
Completeness [%]	96.9	94.7
Redundancy	2.6	1.06

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4 *	298	0.2 M acetate/KOH, 8.6% PEG6000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	16 (mg/ml)
2	1	drop	MES/KOH	40 (mM)
3	1	drop	dithiothreitol	5 (mM)
4	1	reservoir	succinate/KOH	0.1 (M)
5	1	reservoir	PEG6000	8.6 (%(w/v))