1EV7
CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-01-01 |
| Detector | BRANDEIS - B1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.780, 56.160, 59.050 |
| Unit cell angles | 90.00, 95.92, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.380 |
| Rwork | 0.237 |
| R-free | 0.28680 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.055 | 0.185 |
| Total number of observations | 68874 * | |
| Number of reflections | 25755 | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 88.1 | 73.7 |
| Redundancy | 2.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.1 | 4 * | 20 mM malaic acid, 20 mM NaCl, 5 mM CaCl2, 1 mM 2-mercaptoethanol, 2% PEG3350, 1.5% ethanol, pH 6.1, MICRODIALYSIS, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | malaic acid | 20 (mM) | |
| 2 | 1 | 1 | 20 (mM) | ||
| 3 | 1 | 1 | 5 (mM) | ||
| 4 | 1 | 1 | beta-mercaptoethanol | 1 (mM) | |
| 5 | 1 | 1 | PEG3350 | 2 (%) | |
| 6 | 1 | 1 | ethanol | 1.5 (%) |
