1EV6
Structure of the monoclinic form of the M-cresol/insulin R6 hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-18 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.247, 61.739, 47.467 |
| Unit cell angles | 90.00, 111.32, 90.00 |
Refinement procedure
| Resolution | 20.950 - 1.900 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.23500 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 20.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.950 | 1.940 |
| High resolution limit [Å] | 1.800 | 1.890 |
| Rmerge | 0.039 | 0.287 |
| Total number of observations | 174925 * | |
| Number of reflections | 28441 | |
| <I/σ(I)> | 40.6 | 5.4 |
| Completeness [%] | 92.6 | 92.6 |
| Redundancy | 6.15 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 298 | 30 mg insulin, 3.0 ml 0.02 M HCl, 0.3 ml 0.15 M Zinc Acetate, 1.5 ml 0.2 M Sodium Citrate, 1.2 ml 5% m-Cresol in ethanol, pH 6.5, SLOW COOLING, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | insulin | 30mg | |
| 2 | 1 | 1 | 0.02 (M) | 3.0ml | |
| 3 | 1 | 1 | zinc acetate | 0.15 (M) | 0.3ml |
| 4 | 1 | 1 | sodium citrate | 0.2 (M) | 1.5ml |
| 5 | 1 | 1 | m-cresol in ethanol | 5 (%) | 1.2ml |
