1EV3
Structure of the rhombohedral form of the M-cresol/insulin R6 hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-13 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.866, 78.866, 39.465 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.720 - 1.780 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.26600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 20.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.720 | 1.830 |
| High resolution limit [Å] | 1.700 | 1.780 |
| Rmerge | 0.047 | 0.173 |
| Total number of observations | 64359 * | |
| Number of reflections | 9334 | |
| <I/σ(I)> | 60.6 | 10 |
| Completeness [%] | 92.8 | 82.6 |
| Redundancy | 6.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.5 | 298 | 30 mg insulin, 3.0 ml of 0.02 M HCl, 0.3 ml of 0.15 M Zinc Acetate, 1.5 ml of 0.2 M Sodium Citrate, 1.2 ml of 2.5% m-cresol in acetone, 0.36 gm sodium chloride, pH 8.5, SLOW COOLING, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | insulin | 30mg | |
| 2 | 1 | 1 | 0.02 (M) | 3.0ml | |
| 3 | 1 | 1 | zinc acetate | 0.15 (M) | 0.3ml |
| 4 | 1 | 1 | sodium citrate | 0.2 (M) | 1.5ml |
| 5 | 1 | 1 | m-cresol in acetone | 2.5 (%) | 1.2ml |
| 6 | 1 | 1 | 0.36g |
