1EUZ
GLUTAMATE DEHYDROGENASE FROM THERMOCOCCUS PROFUNDUS IN THE UNLIGATED STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.480, 163.120, 132.190 |
| Unit cell angles | 90.00, 113.20, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.250 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.640 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.063 * | 0.322 * |
| Total number of observations | 1488939 * | |
| Number of reflections | 206382 | |
| <I/σ(I)> | 17.9 | 3.4 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 7.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG8000, Lithium sulfate, sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 6.4 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 1.0 (%(w/v)) | |
| 3 | 1 | reservoir | lithium sulfate | 1.5 (M) | |
| 4 | 1 | reservoir | sodium acetate | 50 (mM) |
