1EUZ

GLUTAMATE DEHYDROGENASE FROM THERMOCOCCUS PROFUNDUS IN THE UNLIGATED STATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1999-03-15
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	112.480, 163.120, 132.190
Unit cell angles	90.00, 113.20, 90.00

Refinement procedure

Resolution	8.000 - 2.250
R-factor	0.187
Rwork	0.187
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.640
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.063 *	0.322 *
Total number of observations	1488939 *
Number of reflections	206382
<I/σ(I)>	17.9	3.4
Completeness [%]	99.7	99.4
Redundancy	7.21

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	PEG8000, Lithium sulfate, sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	6.4 (mg/ml)
2	1	reservoir	PEG8000	1.0 (%(w/v))
3	1	reservoir	lithium sulfate	1.5 (M)
4	1	reservoir	sodium acetate	50 (mM)