1EUT
SIALIDASE, LARGE 68KD FORM, COMPLEXED WITH GALACTOSE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.280, 117.300, 60.240 |
| Unit cell angles | 90.00, 96.17, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.570 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.500 * | 2.500 * |
| Rmerge | 0.039 | 0.092 * |
| Total number of observations | 49584 * | |
| Number of reflections | 20112 | |
| Completeness [%] | 79.2 | 76.8 * |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | Taylor, G., (1992) J.Mol.Biol., 225, 1135. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 8 (%) |
