1EUN
STRUCTURE OF 2-KETO-3-DEOXY-6-PHOSPHOGLUCONATE ALDOLASE FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 1999-10-04 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.950, 85.210, 133.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.490 - 2.000 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.25900 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.130 |
| High resolution limit [Å] | 2.000 * | 2.000 |
| Rmerge | 0.075 * | 0.269 |
| Number of reflections | 39315 * | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 94.8 * | 79.4 |
| Redundancy | 4.66 | 4.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 320 | PEG 3350, Ammonium sulfate, Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 320K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | PEG3500 | 18.5 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
