1EUD

CRYSTAL STRUCTURE OF PHOSPHORYLATED PIG HEART, GTP-SPECIFIC SUCCINYL-COA SYNTHETASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	DIFFRACTOMETER
Collection date	1995-03-01
Detector	WEISSENBERG
Spacegroup name	P 1 21 1
Unit cell lengths	86.907, 82.278, 50.017
Unit cell angles	90.00, 104.37, 90.00

Refinement procedure

Resolution	41.000 - 2.100
R-factor	0.162
Rwork	0.156
R-free	0.21500
RMSD bond length	0.018
RMSD bond angle	1.900
Data reduction software	WEIS
Data scaling software	WEIS
Phasing software	TNT
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000	2.130
High resolution limit [Å]	2.100	2.090
Rmerge	0.088	0.358
Total number of observations	253330 *
Number of reflections	39063
Completeness [%]		92.6
Redundancy	4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS		4 *	sodium,potassium(1:3) phosphate, 2-mercaptoethanol, ammonium sulfate, MICRODIALYSIS, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	11-14 (mg/ml)
2	1	2	ammonium sulfate	1.65-1.68 (M)
3	1	2	2-mercaptoethanol	1 (mM)
4	1	2	Na/K(1:3)phosphate	43 (mM)