1EUA
SCHIFF BASE INTERMEDIATE IN KDPG ALDOLASE FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 190 |
| Detector technology | CCD |
| Collection date | 2000-03-20 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.410, 85.720, 133.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.370 - 1.950 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.24300 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.087 | 0.293 |
| Number of reflections | 44409 | |
| <I/σ(I)> | 30 | |
| Completeness [%] | 99.4 * | 92.2 |
| Redundancy | 11.1 | 4.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 293 | PEG 4000, ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | PEG3500 | 18.5 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
