1EU3
CRYSTAL STRUCTURE OF THE SUPERANTIGEN SMEZ-2 (ZINC BOUND) FROM STREPTOCOCCUS PYOGENES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1999-02-28 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 148.230, 39.960, 104.070 |
Unit cell angles | 90.00, 134.05, 90.00 |
Refinement procedure
Resolution | 37.050 - 1.680 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.23000 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.740 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.039 * | 0.232 * |
Total number of observations | 537930 * | |
Number of reflections | 49704 * | |
<I/σ(I)> | 38.1 | |
Completeness [%] | 95.4 * | 84.7 * |
Redundancy | 5.5 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 18 * | PEG 5000 MME, cacodylate, sodium phosphate, Zinc acetate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | phosphate | 50 (mM) | |
3 | 1 | reservoir | cacodylate/KOH | 200 (mM) | |
4 | 1 | reservoir | PEG5000 MME | 16 (%) |