1ETY

THE CRYSTAL STRUCTURE OF E. COLI WILD-TYPE FIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1999-09-02
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	77.470, 50.360, 47.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.400 - 2.000
Rwork	0.233
R-free	0.27100
Starting model (for MR)	The wild-type FIS
RMSD bond length	0.011
RMSD bond angle	1.082
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.062 *	0.254 *
Total number of observations	70772 *
Number of reflections	13174
<I/σ(I)>	25.1
Completeness [%]	99.7	99.6
Redundancy	5.37	6.39

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	10 mg/ml protein, 0.5 M sodium chloride, 0.1 M sodium acetate, 0.05 M sodium cacodylate(pH 6.5), 15% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		1 (M)
3	1	drop	Tris-HCl	20 (mM)