1ETX

THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT Q74A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1999-09-08
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	77.700, 50.290, 47.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.430 - 1.900
Rwork	0.223
R-free	0.24800
Starting model (for MR)	The wild-type FIS
RMSD bond length	0.009
RMSD bond angle	18.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.073 *	0.559
Total number of observations	85997 *
Number of reflections	15224
<I/σ(I)>	22.7
Completeness [%]	100.0	100
Redundancy	5.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	10 mg/ml protein, 0.5 M sodium chloride, 0.1 M sodium acetate, 0.05 M sodium cacodylate(pH 6.5), 15% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		1 (M)
3	1	drop	Tris-HCl	20 (mM)
4	1	reservoir	sodium acetate	0.2 (M)
5	1	reservoir	sodium cacodylate	0.1 (M)
6	1	reservoir	PEG8000	30 (%)