1ETX
THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT Q74A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-08 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.700, 50.290, 47.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.430 - 1.900 |
| Rwork | 0.223 |
| R-free | 0.24800 |
| Starting model (for MR) | The wild-type FIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 18.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 * | 0.559 |
| Total number of observations | 85997 * | |
| Number of reflections | 15224 | |
| <I/σ(I)> | 22.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10 mg/ml protein, 0.5 M sodium chloride, 0.1 M sodium acetate, 0.05 M sodium cacodylate(pH 6.5), 15% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 1 (M) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 6 | 1 | reservoir | PEG8000 | 30 (%) |
