1ETP
CRYSTAL STRUCTURE OF CYTOCHROME C4 FROM PSEUDOMONAS STUTZERI
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 274 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-03-12 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.490, 58.580, 63.510 |
| Unit cell angles | 90.00, 96.96, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.26700 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 21.700 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.700 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.033 * |
| Number of reflections | 18020 |
| <I/σ(I)> | 13.5 |
| Completeness [%] | 98.1 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 293 * | Kadziola, A., (1995) Acta Crystallogr.,Sect.D, 51, 1071. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 2.5 (mM) | |
| 3 | 1 | drop | 0.05 (M) | ||
| 4 | 1 | drop | ammonium acetate | 0.1 (M) | |
| 5 | 1 | drop | PEG4000 | 15 (%) | |
| 6 | 1 | drop | sodium citrate | 0.05 (M) | |
| 7 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 8 | 1 | reservoir | PEG4000 | 30 (%) | |
| 9 | 1 | reservoir | sodium citrate | 0.1 (M) |
