1ETO

THE CRYSTAL STRUCTURE OF E. COLI FIS MUTANT R71L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1999-08-16
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	46.030, 70.830, 74.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 1.900
Rwork	0.225
R-free	0.26000
Starting model (for MR)	The wild-type FIS
RMSD bond length	0.011
RMSD bond angle	20.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.057 *	0.511 *
Total number of observations	114334 *
Number of reflections	19893
<I/σ(I)>	30.7
Completeness [%]	99.8	100
Redundancy	5.75	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	10 mg/ml protein, 0.5 M sodium chloride, 0.05 M Na-Hepes (pH 7.5), 1.0 M ammonium sulfate, 1% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		1 (M)
3	1	drop	Tris-HCl	20 (mM)
4	1	reservoir	ammonium sulfate	2.0 (M)
5	1	reservoir	PEG400	2 (%)