1ESV

COMPLEX BETWEEN LATRUNCULIN A:RABBIT MUSCLE ALPHA ACTIN:HUMAN GELSOLIN DOMAIN 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	1998-02-28
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	56.750, 69.250, 182.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.700 - 2.000
Rwork	0.228
R-free	0.28600
RMSD bond length	0.021
RMSD bond angle	0.006
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.700	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.066	0.310
Number of reflections	42367
<I/σ(I)>	7.2
Completeness [%]	85.6	68
Redundancy	2.8	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.6	4 *	or 20 degrees centigrade *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG6000	5-9 (%(w/v))