1ESF
STAPHYLOCOCCAL ENTEROTOXIN A
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-08-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.600, 62.500, 75.100 |
| Unit cell angles | 90.00, 96.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.27700 |
| Structure solution method | MIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 26.871 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.044 |
| Number of reflections | 37509 |
| Completeness [%] | 96.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | Tricine | 20 (mM) | |
| 3 | 1 | drop | 0.060 (mM) | ||
| 4 | 1 | reservoir | PEG4000 | 20 (%) | |
| 5 | 1 | reservoir | MES | 250 (mM) |
