1ESD

THE MOLECULAR MECHANISM OF ENANTIORECOGNITION BY ESTERASES

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	131.133, 48.596, 70.353
Unit cell angles	90.00, 118.03, 90.00

Refinement procedure

Resolution	7.500 - 2.300
R-factor	0.18
RMSD bond length	0.021
RMSD bond angle	0.065
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.070 *
Number of reflections	14801 *
Completeness [%]	82.9 *
Redundancy	2.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *		Green, R., (1992) J. Mol. Biol., 227, 569. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium sulfate	45 (%sat)
3	1	drop	potassium phosphate	100 (mM)
4	1	reservoir	ammonium sulfate	45 (%sat)
5	1	reservoir	potassium phosphate	100 (mM)