1ES9
X-RAY CRYSTAL STRUCTURE OF R22K MUTANT OF THE MAMMALIAN BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASES (PAF-AH)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-14 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 81.049, 81.049, 72.662 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.300 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.21700 |
RMSD bond length | 0.020 |
RMSD bond angle | 0.023 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.069 | 0.366 * |
Total number of observations | 461423 * | |
Number of reflections | 85358 * | |
<I/σ(I)> | 32 | |
Completeness [%] | 98.7 * | 90.4 * |
Redundancy | 5.4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.8 | 293 | Ho, Y.S., (1999) Protein Eng., 12, 693. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-9 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 12-18 (%) | |
3 | 1 | reservoir | Tris-HCl |