1ERT

HUMAN THIOREDOXIN (REDUCED FORM)

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1995-02-02
Detector	ENRAF-NONIUS FAST
Spacegroup name	C 1 2 1
Unit cell lengths	67.910, 26.490, 51.690
Unit cell angles	90.00, 95.22, 90.00

Refinement procedure

Resolution	15.000 - 1.700
R-factor	0.21
Rwork	0.210
R-free	0.24000
RMSD bond length	0.015
RMSD bond angle	24.600 *
Data reduction software	MADNES
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000
High resolution limit [Å]	1.700
Rmerge	0.047
Total number of observations	19412 *
Number of reflections	9477 *
Completeness [%]	92.0	78 *
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	3.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium acetate	10 (mM)
2	1	drop	MPD	25 (%(v/v))
3	1	drop	dithiothreitol	5 (mM)
4	1	reservoir	sodium acetate	10 (mM)
5	1	reservoir	MPD	50 (%(v/v))
6	1	reservoir	dithiothreitol	5 (mM)