1ERB

THE INTERACTION OF N-ETHYL RETINAMIDE WITH PLASMA RETINOL-BINDING PROTEIN (RBP) AND THE CRYSTAL STRUCTURE OF THE RETINOID-RBP COMPLEX AT 1.9 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	46.270, 49.110, 76.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.000 * - 1.900
R-factor	0.172
RMSD bond length	0.009
RMSD bond angle	26.600 *
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.038 *
Total number of observations	37176 *
Number of reflections	11262 *	1645 *
Completeness [%]	79.0 *	59 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *			referred to 1hbq *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	bRBP	6-10 (mg/ml)
2	1	2	sodium chloride	0.1 (M)
3	1	2	cadmium acetate	0.01-1. (mM)
4	1	2	sodium acetate	0.1 (M)