1EQY
COMPLEX BETWEEN RABBIT MUSCLE ALPHA-ACTIN: HUMAN GELSOLIN DOMAIN 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.330, 70.880, 183.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.400 - 2.300 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.28000 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.540 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.400 | 2.460 |
| High resolution limit [Å] | 2.300 | 2.330 |
| Rmerge | 0.092 | 0.210 |
| Number of reflections | 28998 | |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 89.1 | 66 |
| Redundancy | 3.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.6 | 20 * | PEG 6000, sodium chloride, adenosine triphosphate, calcium, magnesium, sodium azide, pH 6.6, Vapor Diffusion, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 5-9 (%) | |
| 2 | 1 | reservoir | 0.15 (M) | ||
| 3 | 1 | reservoir | ATP | 0.1 (mM) | |
| 4 | 1 | reservoir | 0.1 (mM) | ||
| 5 | 1 | reservoir | 1 (mM) |
