1EQN
E.COLI PRIMASE CATALYTIC CORE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98636, 0.97939, 0.97900, 0.96526 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.674, 107.626, 263.069 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 - 2.900 |
| R-factor | 0.2259 |
| Rwork | 0.220 |
| R-free | 0.27600 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.414 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.058 | 0.207 |
| Total number of observations | 273234 * | |
| Number of reflections | 40874 * | |
| <I/σ(I)> | 31.3 | |
| Completeness [%] | 97.6 | 90.8 |
| Redundancy | 6.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 20 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 16-19 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 0.053-0.063 (mM) | |
| 4 | 1 | reservoir | sodium acetate | 0.11-0.13 (M) |
