1EQ7

CORE STRUCTURE OF THE OUTER MEMBRANE LIPOPROTEIN FROM ESCHERICHIA COLI AT 1.9 ANGSTROM RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1999-03-19
Detector	RIGAKU RAXIS IV
Spacegroup name	H 3 2
Unit cell lengths	34.363, 34.363, 194.135
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.214
Rwork	0.214
R-free	0.26900
RMSD bond length	0.006
RMSD bond angle	17.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	1.900
Rmerge	0.055
Number of reflections	3749
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	drop was eluted 1:1 with a reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	0.2 (M)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	PEG4000	30 (%)