1EPV
ALANINE RACEMASE WITH BOUND INHIBITOR DERIVED FROM D-CYCLOSERINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.157, 90.243, 85.261 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.200 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.23100 * |
| RMSD bond length | 0.020 |
| RMSD bond angle | 25.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.084 | 0.339 |
| Total number of observations | 218570 * | |
| Number of reflections | 39472 * | |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 90.8 | 92.3 |
| Redundancy | 5.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, sodium acetate, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 22 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 23 (%(w/v)) | |
| 3 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 4 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
