1EPU
X-RAY crystal structure of neuronal SEC1 from squid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-02-07 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.063, 121.989, 69.046 |
Unit cell angles | 90.00, 104.53, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.400 |
R-factor | 0.2508 |
Rwork | 0.240 |
R-free | 0.27700 |
Structure solution method | TWO-WAVELENGTH MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 22.360 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.055 | 0.305 |
Total number of observations | 116511 * | |
Number of reflections | 34634 | |
<I/σ(I)> | 11.1 | 2.4 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 * | 277 | Bracher, A., (2000) Acta Crystallogr., Sect.D, 56, 501. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG1000 | 15 (%(w/v)) | |
2 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
4 | 1 | reservoir | dithiothreitol | 20 (mM) | |
5 | 1 | reservoir | 10 (mM) |