1EP3

CRYSTAL STRUCTURE OF LACTOCOCCUS LACTIS DIHYDROOROTATE DEHYDROGENASE B. DATA COLLECTED UNDER CRYOGENIC CONDITIONS.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1999-04-26
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	199.890, 199.890, 80.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.195 *
Rwork	0.195
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 1EP1
RMSD bond length	0.009
RMSD bond angle	23.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.073	0.327
Total number of observations	270295 *
Number of reflections	35821
<I/σ(I)>	16.62
Completeness [%]	98.7	87.5
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6 *	298	Rowland, P., (1997) Acta Crystallogr., Sect.D, 53, 802. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium phosphate	50 (mM)
3	1	drop	glycerol	10 (%)
4	1	reservoir	ammonium sulfate	2.4 (M)
5	1	reservoir	sodium acetate	0.1 (M)