1EOH

GLUTATHIONE TRANSFERASE P1-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 21 21
Unit cell lengths	82.970, 84.020, 236.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
Rwork	0.231
R-free	0.28640
RMSD bond length	0.009 *
RMSD bond angle	1.300 *
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.500 *
Rmerge	0.115 *	0.396 *
Total number of observations	231441 *
Number of reflections	57974 *
Completeness [%]	99.0 *	58 *
Redundancy	4.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.0 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	reservoir	ammonium sulfate	20-25 (%(w/v))
4	1	reservoir	dithiothreitol	60 (mM)
5	1	reservoir	MES	100 (mM)