1EO4
ECORV BOUND TO MN2+ AND COGNATE DNA CONTAINING A 3'S SUBSTITION AT THE CLEAVAGE SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.700, 48.500, 63.700 |
| Unit cell angles | 96.70, 109.60, 106.30 |
Refinement procedure
| Resolution | 4.900 - 1.900 |
| R-factor | 0.213 * |
| Rwork | 0.213 |
| R-free | 0.30500 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.010 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | 0.362 |
| Total number of observations | 72080 * | |
| Number of reflections | 36838 | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 96.5 | 95 |
| Redundancy | 1.96 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 17 * | 25% PEG 4000, 0.1M HEPES, 0.15M NaCl, 50 mM MnCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | MnCl2 | ||
| 4 | 1 | 1 | PEG 4000 | ||
| 5 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | EcoRV | 10 (mg/ml) | |
| 2 | 1 | drop | DNA | 1.0 (mg/ml) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | |
| 4 | 1 | reservoir | 150 (mM) | ||
| 5 | 1 | reservoir | PEG4000 | 10-15 (%) | |
| 6 | 1 | reservoir | 50 (mM) |
