1EO2
CRYSTAL STRUCTURE OF ACINETOBACTER SP. ADP1 PROTOCATECHUATE 3,4-DIOXYGENASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1999-01-01 |
Detector | SIEMENS HI-STAR |
Spacegroup name | I 2 3 |
Unit cell lengths | 145.500, 145.500, 145.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.250 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.21800 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.480 |
Data scaling software | X-GEN |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.074 | 0.322 |
Number of reflections | 23393 | |
<I/σ(I)> | 8.96 | |
Completeness [%] | 98.6 | 92.3 |
Redundancy | 6.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 18 * | 100mM Tris-HCl pH 7.5, 2.0 M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
3 | 1 | reservoir | Tris-HCl | 50 (mM) |