1ENS

CRYSTALS OF DEMETALLIZED CONCANAVALIN A SOAKED WITH COBALT HAVING A COBALT ION BOUND IN THE S1 SITE

Experimental procedure

Detector technology	DIFFRACTOMETER
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 2
Unit cell lengths	61.350, 86.300, 91.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.207
Rwork	0.207
R-free	0.25940 *
RMSD bond length	0.019
RMSD bond angle	27.980 *
Data reduction software	MADNES
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.700
Rmerge	0.080
Total number of observations	31839 *
Number of reflections	12911
Completeness [%]	93.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5		Bouckaert, J., (1995) Proteins: Struct.,Funct., Genet., 23, 510. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	2 (M)
2	1	reservoir	sodium acetate	100 (mM)
3	1	drop	protein	11 (mg/ml)
4	1	drop	PEG6000	5 (%)