1ENP
BRASSICA NAPUS ENOYL ACP REDUCTASE/NADH BINARY COMPLEX AT PH 8.0 AND ROOM TEMPERATURE
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1995-04-11 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 70.500, 70.500, 117.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.900 |
| Data reduction software | SDMS |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Rmerge | 0.043 |
| Total number of observations | 58714 * |
| Number of reflections | 8364 |
| Completeness [%] | 88.0 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 * | 17 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 10 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | nucleotide | 0.15 (M) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 6 | 1 | reservoir | HEPES/NaOH | 0.1 (M) |
