1EN7
ENDONUCLEASE VII (ENDOVII) FROM PHAGE T4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.990, 39.450, 75.750 |
| Unit cell angles | 90.00, 106.20, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| Rwork | 0.239 |
| R-free | 0.30800 |
| Structure solution method | SIRAS |
| Starting model (for MR) | ENDOVII N62D MUTANT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 0.032 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.281 | 2.460 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.060 * | 0.253 * |
| Number of reflections | 17578 | |
| <I/σ(I)> | 11.8 | 3 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 4 * | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaCl | ||
| 10 | 1 | 2 | 2-MERCAPTO-ETHANOL | ||
| 11 | 1 | 2 | TRIS | ||
| 12 | 1 | 2 | NaN3 | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | ZnCl2 | ||
| 4 | 1 | 1 | 2-MERCAPTO-ETHANOL | ||
| 5 | 1 | 1 | GLYCEROL | ||
| 6 | 1 | 1 | MOPS | ||
| 7 | 1 | 2 | PEG 5000 | ||
| 8 | 1 | 2 | CaCl2 | ||
| 9 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | mPEG2000 | 16.5-20 (%) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | 5 (mM) | ||
| 6 | 1 | reservoir | ammonium sulfate | 5 (mM) | |
| 7 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) |
