1EMR
CRYSTAL STRUCTURE OF HUMAN LEUKEMIA INHIBITORY FACTOR (LIF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-01-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.500, 45.300, 77.700 |
| Unit cell angles | 90.00, 112.30, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| Rwork | 0.191 |
| R-free | 0.29300 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.250 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.500 |
| Rmerge | 0.035 |
| Number of reflections | 2728 |
| Completeness [%] | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 200 mM sodium acetate, 100 mM cacodylate buffer, 30% w/v PEG 8000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
