1EMM

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1996-07-25
Detector	MAC Science DIP-2000
Spacegroup name	P 61 2 2
Unit cell lengths	89.400, 89.400, 129.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.194
Rwork	0.194
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.070 *	0.351 *
Number of reflections	12098
Completeness [%]	87.1	71.2
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5		protein solution is mixed in a 1:1 ratio with well solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	ammonium sulfate	1.95 (M)
4	1	reservoir	Tris-HCl	100 (mM)